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FALSE CLEAVERS
(
Galium spurium
)
with
GROUP B/2 resistance: (INHIBITION OF ACETOLACTATE SYNTHASE )
Inhibition of Acetolactate Synthase
MUTATION: TRYPTOPHAN 574 to LEUCINE
False Cleavers
(
Galium spurium
) is a dicot plant in the rubiaceae family. A single amino acid substitution from Tryptophan 574 to Leucine has led to resistance to Inhibition of Acetolactate Synthase as indicated in the table below.
False Cleavers
Chemical Family
Example Herbicide
Resistance Level
Imidazolinones
Imazethapyr
Resistant > 10 fold
Pyrimidinyl benzoates
Bispyribac-Na
Not Determined
Sulfonylureas
Chlorsulfuron
Not Determined
Triazolopyrimidine - Type 1
Chloransulam-methyl
Resistant > 10 fold
Triazolinones
Flucarbazone-Na
Not Determined
REFERENCES
Beckie, H. J. ; Warwick, S. I. ; Sauder, C. A. ; Kelln, G. M. ; Lozinski, C.
.
2012
.
Acetolactate synthase inhibitor-resistant false cleavers (
Galium spurium
) in western Canada
.
Weed Technology
26
:
151 - 155
.
Cleavers species (false cleavers and catchweed bedstraw) are among the top 10 most abundant weeds across the prairie region of western Canada, and are increasing in relative abundance at the fastest rate since the 1970s. In 2008, two false cleavers populations from Tisdale and Choiceland, Saskatchewan, were suspected of acetolactate synthase (ALS)-inhibitor resistance. Dose-response experiments were conducted with the use of imazethapyr and florasulam, both ALS inhibitors, as well as fluroxypyr, a synthetic auxin. Additionally, a 1,954-base-pair region of the
ALS
gene including sites known to confer
ALS
resistance were sequenced. Both populations were highly resistant to imazethapyr (resistance factors greater than 100), one population (Tisdale) was highly resistant to florasulam (Choiceland population susceptible, although a second, larger screening of 200 individuals indicated low frequency [2%] florasulam resistance), and both populations were susceptible to fluroxypyr. All sequenced Tisdale individuals screened with imazethapyr posessed the Trp
574
Leu mutation. In contrast, three point mutations were found for Choiceland individuals sequenced: Ser
653
Asn, Trp
574
Leu, and Asp
376
Glu. These
ALS
target-site mutations have not been documented previously in this species.
.
This case was entered by Patrick Tranel Email:
tranel@illinois.edu
PERMISSION MUST BE OBTAINED FIRST if you intend to base a significant portion of a scientific paper on data derived from this site.
Cite this site as:
Heap, I. The International Survey of Herbicide Resistant Weeds. Online. Internet.
Saturday, September 7, 2024
. Available
www.weedscience.org
Copyright © 1993-
2024
WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.
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