<b>HRAC Group 9</b> <font size='2'> (Legacy G) </font> resistant Conyza canadensis from Mexico

INTERNATIONAL HERBICIDE-RESISTANT WEED DATABASE

HRAC GROUP 9 (LEGACY G) RESISTANT HORSEWEED
(Conyza canadensis)

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G)

Mexico, Veracuz

INTRODUCTION		HORSEWEED
Horseweed (Conyza canadensis) is a dicot weed in the Asteraceae family. In Mexico this weed first evolved resistance to Group 9 (Legacy G) herbicides in 2018 and infests Grapefruit, Lime, sour, and Oranges. Group 9 (Legacy G) herbicides are known as Inhibition of Enolpyruvyl Shikimate Phosphate Synthase (Inhibition of EPSP synthase). Research has shown that these particular biotypes are resistant to glyphosate and they may be cross-resistant to other Group 9 (Legacy G) herbicides. The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

QUIK STATS (last updated Apr 24, 2025 )
Common Name	Horseweed
Species	Conyza canadensis
Group	Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G)
Herbicides	glyphosate
Location	Mexico, Veracuz
Year	2018
Situation(s)	Grapefruit, Lime, sour, and Oranges
Contributors - (Alphabetically)	Rafael De Prado, Ja Dominguez Valenzuela, Candelario Palma-Bautista, and José G. Vázquez-García
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NOTES ABOUT THIS BIOTYPE

GENERAL

Ja Dominguez Valenzuela

Journal of Agricultural and Food Chemistry Vol 71/Issue 11

Agricultural and Environmental Chemistry
March 9, 2023 Reduced Glyphosate Movement and Mutation of the EPSPS Gene (Pro106Ser) Endow Resistance in Conyza canadensis Harvested in Mexico

Candelario Palma-Bautista
José G. Vázquez-Garcia
Gabriela López-Valencia
José Alfredo Domínguez-Valenzuela
Francisco Barro
Rafael De Prado

Abstract Glyphosate has been the most widely used herbicide for decades providing a unique tool, alone or in mixtures, to control weeds on citrus in Veracruz. Conyza canadensis has developed glyphosate resistance for the first time in Mexico. The level and mechanisms of resistance of four resistant populations Rs (R1, R2, R3, and R4) were studied and compared with that of a susceptible population (S). Resistance factor levels showed two moderately resistant populations (R2 and R3) and two highly resistant populations (R1 and R4). Glyphosate translocation through leaves to roots was ∼2.8 times higher in the S population than in the four R populations. A mutation (Pro106Ser) in the EPSPS2 gene was identified in the R1 and R4 populations. Mutation in the target site associated with reduced translocation is involved in increased glyphosate resistance in the R1 and R4 populations; whereas for the R2 and R3 populations, it was only mediated by reduced translocation. This is the first study of glyphosate resistance in C. canadensis from Mexico in which the resistance mechanisms involved are described in detail and control alternatives are proposed.

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ACADEMIC ASPECTS

Confirmation Tests

Greenhouse, and Laboratory trials comparing a known susceptible Horseweed biotype with this Horseweed biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.

Genetics

Genetic studies on HRAC Group 9 resistant Horseweed have not been reported to the site. There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature

Mechanism of Resistance

Studies on the mechanism of resistance of Group 9 (Legacy G) resistant Horseweed from Mexico indicate that resistance is due to an altered target site. There may be a note below or an article discussing the mechanism of resistance in the Fact Sheets and Other Literature

Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes. If you have any information pertaining to the fitness of Group 9 (Legacy G) resistant Horseweed from Mexico please update the database.

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CONTRIBUTING WEED SCIENTISTS

RAFAEL DE PRADO

Universidad De Cordoba Qvimica Agricola Y Edafologa E T S Ingenieros Agronomos PO Box 119 Cordoba, E-14080, Andalusia Spain Email Rafael De Prado

JA DOMINGUEZ VALENZUELA

Phd Universidad Autónoma Chapingo Parasitología Agrícola Area De Malezas Departamento De Parasitología Agricola Texcoco, 56230, Andalusia Mexico Email Ja Dominguez Valenzuela

CANDELARIO PALMA-BAUTISTA

Investigador Posdoctoral Universidad Autónoma Chapingo Departamento de Parasitología Agrícola Campus Central Carretera México – Texcoco Km 38.5 Texcoco, México CP 56230 Texcoco, 56230, Estado de México Mexico Email Candelario Palma-Bautista

JOSÉ G. VÁZQUEZ-GARCÍA

Investigador Posdoctoral University of Cordoba Agricultural Chemistry and Edaphology Campus of Rabanales Cordoba, 14071, Province Spain Email José G. Vázquez-García

ACKNOWLEDGEMENTS
The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Mexico have been instrumental in providing you this information. Particular thanks is given to Rafael De Prado, Ja Dominguez Valenzuela, Candelario Palma-Bautista, and José G. Vázquez-García for providing detailed information.

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Herbicide Resistant Horseweed Globally
(Conyza canadensis)

Country

FirstYear

Situation

Active Ingredients

Site of Action

Belgium

1989

Corn (maize), Nurseries, and Roadsides

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Belgium

1998

Nurseries

paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

Brazil

2005

Fruit, Orchards, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Canada (Ontario)

1993

Peaches

paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

Canada (Ontario)

2010

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Canada (Ontario)

2011

Soybean

cloransulam-methyl, and glyphosate

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

China

2006

Orchards

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Czech Republic

1987

Corn (maize), Nurseries, Orchards, Pastures, Railways, and Roadsides

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Czech Republic

2007

Railways

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

France

1981

Cropland

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

France

1988

Orchards

linuron

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

France

2019

Grapes

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Greece

2012

Grapes, and Orchards

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Hungary

2016

Grapes

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Israel

1993

Forests, Industrial sites, and Roadsides

atrazine, chlorsulfuron, imazapyr, metribuzin, pyrithiobac-sodium, and sulfometuron-methyl

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Italy

2011

Orchards

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Japan

1980

Grapes, Orchards, Railways, and Roadsides

paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

Japan

2014

Railways

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Mexico

2018

Grapefruit, Lime, sour, and Oranges

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Poland

1983

Orchards

simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Poland

2000

Railways

imazapyr

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Poland

2010

Railways

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Portugal

2011

Olive

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

South Korea

2017

Orchards

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Spain

1987

Corn (maize), Orchards, and Roadsides

atrazine, and simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Spain

2006

Orchards

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Switzerland

1982

Corn (maize), Nurseries, Orchards, Pastures, Railways, and Roadsides

atrazine, and simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Switzerland

2021

Grapes

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United Kingdom

1982

Orchards

simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Mississippi)

1994

Soybean

paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

United States (Delaware)

2000

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Tennessee)

2001

Cotton, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Ohio)

2001

Soybean

chlorimuron-ethyl, and cloransulam-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Kentucky)

2001

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Ohio)

2002

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Indiana)

2002

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Maryland)

2002

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (New Jersey)

2002

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Michigan)

2002

Corn (maize), and Soybean

chlorimuron-ethyl, and cloransulam-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Michigan)

2002

Blueberries

atrazine, diuron, and simazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Michigan)

2002

Blueberries

diuron

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Missouri)

2002

Corn (maize), Cotton, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Ohio)

2003

Soybean

chlorimuron-ethyl, cloransulam-methyl, and glyphosate

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Arkansas)

2003

Cotton

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Mississippi)

2003

Corn (maize), Cotton, Rice, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (North Carolina)

2003

Cotton

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Delaware)

2003

Soybean

paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

United States (Pennsylvania)

2003

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (California)

2005

Corn (maize), Fencelines, Grapes, Orchards, Roadsides, and Wheat

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Illinois)

2005

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Kansas)

2005

Cotton, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Virginia)

2005

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Indiana)

2005

Soybean

chlorimuron-ethyl, and cloransulam-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Nebraska)

2006

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Michigan)

2007

Nurseries

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Mississippi)

2007

Soybean

glyphosate, and paraquat

Multiple Resistance: 2 Sites of Action
PS I Electron Diversion ( HRAC Group 22 (Legacy D)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (West Virginia)

2007

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Oklahoma)

2009

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (South Dakota)

2010

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Delaware)

2010

Soybean, and Wheat

glyphosate, thifensulfuron-methyl, and tribenuron-methyl

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Iowa)

2011

Corn (maize), and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Kansas)

2011

Corn (maize), Cotton, Soybean, and Wheat

chlorsulfuron, iodosulfuron-methyl-Na, metsulfuron-methyl, rimsulfuron, thiencarbazone-methyl, thifensulfuron-methyl, and tribenuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Alabama)

2013

Corn (maize), Cotton, and Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Wisconsin)

2013

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Illinois)

2013

Soybean

cloransulam-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (California)

2014

Almonds

glyphosate, and paraquat

Multiple Resistance: 2 Sites of Action
PS I Electron Diversion ( HRAC Group 22 (Legacy D)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Montana)

2015

Roadsides

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Oklahoma)

2018

Cotton, Soybean, and Wheat

chlorsulfuron, and metsulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Oregon)

2023

Fallow, and Wheat

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (New York)

2024

Apples, and Grapes

diquat, and paraquat

PS I Electron Diversion ( HRAC Group 22 (Legacy D)

Literature about Similar Cases

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Page, E., C. Granger, F. Tardif, I. Rajcan, M. Laforest, and R.E. Nurse. 2017. A chrono-geographical assessment of Canadian populations of Conyza canadensis. WSSA Proceedings 57 : 259 - .

In the decade since the introduction of herbicide tolerant (HT) crops, an increasing number of weed species have been identified with resistance to the widely utilized herbicide, glyphosate. In the USA, there are now 14 weed species with confirmed resistance to the non-selective herbicides utilized in these systems. Similarly in Canada, there are 5 weed species that exhibit glyphosate resistance (GR); two of which were documented in the last year alone. Conyza canadensis has developed GR in both countries and its capacity for long-distance dispersal raises the potential of cross-border movement of herbicide resistant traits. The objective of this research was to examine the origins of GR C. canadensis populations from SW Ontario and explore their mechanism(s) of resistance. New and historic populations of C. canadensis collected throughout the counties comprising the most SW portion of the province of Ontario, as well as from bordering states of the USA. Eight microsatellite markers were used to characterize the relatedness of these populations and the target site gene for glyphosate (EPSPS2) was also sequenced. Dose-response studies were carried out to ascertain or confirm the resistance levels of the various populations. Results from the microsatellite and dose response studies indicate that the majority of Canadian GR populations are distinct from the American populations tested and exhibit notably higher resistance. Sequencing of EPSPS2 confirmed that, while GR in American populations is mediated by non-target site mechanisms, the predominate mechanism of resistance in Canadian populations of C. canadensis is target site mediated. These results represent the first report of target site mediate resistance to glyphosate in C. canadensis and clearly expose geographically distinct mechanisms of resistance to a given active ingredient on a regional scale within a single highly mobile species..

Matzrafi, M., T. W. Lazar, M. Sibony, and B. Rubin. 2015. Conyza species: distribution and evolution of multiple target-site herbicide resistances. Planta 242 : 259 - 267.

Conyza canadensis (CC) and Conyza bonariensis (CB) are troublesome weeds around the world. Extensive use of herbicides has led to the evolution of numerous Conyza spp. herbicide-resistant populations. Seeds of 91 CC and CB populations were collected across Israel. They were mostly found (86 %) in roadsides and urban habitats, two disturbed habitats that had been dramatically impacted by human activities, thus we classify these species as anthropogenic. Although pyrithiobac-sodium was only used in cotton fields, 90 % of Conyza spp. populations were identified as pyrithiobac-sodium resistant, suggesting possible natural resistance to pyrithiobac-sodium. CC21 and CC17 C. canadensis populations were highly resistant to all tested ALS inhibitors due to a substitution in the ALS gene from Trp574 to Leu. They were also atrazine resistant due to a substitution in the psbA gene from Ser264 to Gly. The high level of imazapyr and pyrithiobac-sodium resistance observed in the CC10 population was due to an Ala205 to Val substitution. However, high resistance to sulfometuron methyl and pyrithiobac-sodium in population CC6 was due to a point mutation at Pro197 to Ser. All resistant plants of CC21 population showed both psbA (Ser264 to Gly) and ALS (Trp574 to Leu) substitutions, leading us to the conclusion that the attempt to overcome resistance to one mode of action by overuse of another will most likely lead to multiple herbicide resistance. Furthermore, we concluded that only individuals that carry both mutations could survive the shift between the two modes of action and overcome the fitness cost associated with the PSII resistance..

Ford, L. ; Soltani, N. ; McFadden, A. ; Nurse, R. E. ; Robinson, D. E. ; Sikkema, P. H.. 2014. Control of Canada fleabane (Conyza canadensis) with glyphosate DMA/2,4-D choline applications in corn (Zea mays). Agricultural Sciences 5 : 77 - 83.

Glyphosate resistant (GR) Canada fleabane (horseweed) has quickly spread across southwestern Ontario and is a difficult weed to control in GR crops. Glyphosate dimethylamine (DMA)/2,4-D choline (Enlist Duo®^TM Dow AgroSciences LLC), a new herbicide premix developed by Dow Agro Sciences, provides control of GR and other problematic weeds. The objective of this study was to compare single and sequential applications of glyphosate DMA/2,4-D choline for the control of GR Canada fleabane in GR corn. Three single applications of glyphosate (DMA)/2,4-D choline (1720 g.ae.ha^-1) were evaluated: (1) preplant (PP) applied to Canada fleabane up to 10 cm diameter/height, (2) postplant 1 (POST 1) applied when Canada fleabane was up to 20 cm tall and (3) postplant 2 (POST 2) applied up to 30 cm tall Canada fleabane. Four sequential applications were also examined:(1) PP followed by (fb) POST 1, (2) PP fb POST 2, (3) POST 1 fb POST 2 and (4) PP fb POST 1 fb POST 2. The single applications provided 69%-86% control of the GR Canada fleabane while the sequential applications increased control to 92%-100%. Three applications did not provide an increase in control over a sequential two-pass application at 8 weeks after the application (WAA). Results from this research indicate that a sequential 2-pass application of glyphosate DMA/2,4-D choline provided acceptable control of GR Canada fleabane in corn..

Fernando Machado dos Santos , Leandro Vargas , Pedro Jacob Christoffoleti , Franciele Mariani. 2013. Evaluation of horseweed (Conyza sumatrensis) resistance to glyphosate in Rio Grande do Sul – Brazil. Abstract from Doctorate Dissertation : .

(Conyza bonariensisandConyza canadensis) recorded in Rio Grande do Sul State, however resistance ofConyza sumatrensisto glyphosate is registered only in Paraná State. This report has the objective of stating the presence ofConyza sumatrensisresistant to glyphosate biotypes in Rio Grande do Sul State Brazil. To support this report, researches to evaluate the occurrence of glyphosate resistance in horseweed biotypes, in Rio Grande do Sul State, and for that, seed samples were collected in soybean areas with unsatisfactory control, 25 biotypes. These biotypes were preliminary evaluated for resistance to glyphosate, applying a dose of 720 g/ha. Seeds from this first test of two selected biotypes of horseweed, which were not controlled in the first trial, with contrasting degree of susceptibility, were chosen to develop the second trial. The selected biotypes were grown after herbicide application (first trial) to seed production. It was also made, from these biotypes, herbarium specimens, which were sent to the Department of Biology of the Federal University of Santa Maria, where it was cataloged and classified by Dr. Thais Scotti Canto Dorow, plant systematic specialist, asConyza sumatrensis(Retz.) E. Walker (TELES; BORGES; HEIDEN, 2013) and subsequently deposited in the herbarium, SMDM under numbers SMDM 13950, 13951, 13952 and 13953. The biotypes selected, derived from seeds produced were evaluated by dose-response curves, using eight herbicide doses (0, 45, 90, 180, 360, 720, 1,440 and 2,880 g/ha) applied at growth stage of 3 to 4 leaves and/or 0.5 to 1.0 inch height. The experiments were conducted in a greenhouse in a completely randomized arrangement of treatments with four replications. The results indicate that the susceptible biotype to glyphosate has reduced 50 % of dry matter when used dose of 34.3 g/ha, compared with the resistant biotype, that requires 143.6 g/ha for the same control. The dose that provides 50% control of the susceptible population was 172 g/ha, compared with the resistant biotype that needs 2,028 g/ha for the same control, showing a resistance factor of 11.8..

Pavlovic, D. ; Reinhardt, C. F. ; Bozic, D. ; Vrbnicanin, S.. 2013. Determination of Conyza canadensis levels of sensitivity to glyphosate trimesium sulphosate. International Journal of Agriculture and Biology 15 : 1091 - 1097.

Changes in leaf anatomy were examined in two S. African populations of Conyza canadensis of which one was presumed to be resistant (CCPR) and the other susceptible (CCS) to glyphosate. Glyphosate was applied to plants, which were grown from seed collected from these populations, at rates of 1, 2 and 4 kg a.e. ha^-1 of TOUCHDOWN® [active substance: glyphosate trimesium salt, 500 g L^-1] that are equivalent to 2, 4 and 8 L ha^-1 of the herbicide Touchdown. Leaf samples for the light microscope (LM) analysis were collected 3, 7 and 24 h after treatment (HAT). Changes in chlorophyll and shikimate content of leaf material were also examined. Changes in the palisade and pith tissue of leaves were not detected in the investigated populations at 3 and 7 HAT. However, at 24 HAT the different herbicide doses caused changes in leaf anatomy. These changes (injuries) were detected in the CCS at all tested doses, but in the CCPR population of C. canadensis the injuries were observed at only the two highest rates, 2 and 4 kg a.e. ha^-1. Chlorophyll and shikimate contents indicated significant differences between the treated and untreated plants of susceptible population only. Difference in glyphosate resistance between the CCS and CCPR populations was confirmed with an index of resistance of 1.58. This value of the index of resistance indicates that CCS population is 1.58 times more susceptible to glyphosate compared to CCPR population..

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PERMISSION MUST BE OBTAINED FIRST if you intend to base a significant portion of a scientific paper on data derived from this site. Citation:
Heap, I. The International Herbicide-Resistant Weed Database. Online. Thursday, April 24, 2025 . Available www.weedscience.org Copyright © 1993- 2025 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.